✂️ CutSeq
Trim sequencing adapters in a correct way.
Why CutSeq?
NGS library preparation can require many sequential trimming and extraction steps. For example, the SMARTer® Stranded Total RNA-Seq Kit v3 workflow needs at least 9 operations, including adapter removal, UMI extraction, linker masking, and poly-A/T trimming. CutSeq automates these steps in the correct order, reducing errors and saving time.
Key Features
- Single-parameter trimming: Specify your library scheme with one parameter.
- Built-in and custom adapters: Use a preset or define your own scheme.
- Efficient and reproducible: Avoids multiple I/O steps and manual errors.
CutSeq is open source and welcomes contributions! See the GitHub repo for details.
Quick Navigation
- Quick Start - Installation and usage examples
- Adapter Schemes - Comprehensive guide to supported adapter patterns